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cell culture conditions primary human dermal fibroblast normal cells hdfn  (ATCC)


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    ATCC cell culture conditions primary human dermal fibroblast normal cells hdfn
    Cell Culture Conditions Primary Human Dermal Fibroblast Normal Cells Hdfn, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 524 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 524 article reviews
    cell culture conditions primary human dermal fibroblast normal cells hdfn - by Bioz Stars, 2026-06
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    cell culture conditions primary human dermal fibroblast normal cells hdfn  (ATCC)
    99
    ATCC cell culture conditions primary human dermal fibroblast normal cells hdfn
    Cell Culture Conditions Primary Human Dermal Fibroblast Normal Cells Hdfn, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell culture conditions primary human dermal fibroblast normal cells hdfn/product/ATCC
    Average 99 stars, based on 1 article reviews
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    neonatal human fibroblasts  (Lifeline Cell Technology)
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    Staphylococcus epidermidis inhibits fibroblast senescence by inhibiting SASP secretion from UVB-irradiated keratinocytes. (A) Schematic representation of the experimental design. NHEKs were cultured to 90% confluence and then exposed to 10 mJ/cm 2 UVB for induction. Subsequently, 75μg/mL of ≤10kDa S. epi was added to coculture for 48 hours. The conditioned medium containing SASP factors was collected by centrifugation at 2, 000 rpm for 20 minutes, mixed with fresh DMEM at a 1:2 ratio, and the final serum concentration was adjusted to 10%. The mixed medium was used to culture primary human <t>fibroblasts</t> for 48 hours. (B) Fibroblasts were stained with senescence-associated β-gal, and the percentage of senescent cells was quantified by Image (J, C) Protein levels of P16 and P21 in fibroblasts were analyzed by western blotting. Densitometric analysis of protein bands were quantified by Image (J, D) RT-PCR analysis of RNA isolated from fibroblasts was performed to assess the expression of P16, P21 , P53 , TNFα , IL-6 , IL-1β , and MMP1 , with β-actin as the internal control. Con-SASP, SASP collected from control NHEKs; S.epi -SASP, SASP collected from NHEKs treated with 75μg/mL of ≤10kDa S.epi ; UVB-SASP, SASP collected from NHEKs treated with UVB; UVB+ S.epi -SASP, SASP collected from NHEKs treated with UVB and 75μg/mL of ≤10kDa S.epi . Data represent mean ± SEM with n = 3. All the experiments have been repeated three times. Statistical significances were analyzed by One-way ANOVA. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Neonatal Human Fibroblasts, supplied by Lifeline Cell Technology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    normal human neonatal dermal fibroblasts  (ATCC)
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    Staphylococcus epidermidis inhibits fibroblast senescence by inhibiting SASP secretion from UVB-irradiated keratinocytes. (A) Schematic representation of the experimental design. NHEKs were cultured to 90% confluence and then exposed to 10 mJ/cm 2 UVB for induction. Subsequently, 75μg/mL of ≤10kDa S. epi was added to coculture for 48 hours. The conditioned medium containing SASP factors was collected by centrifugation at 2, 000 rpm for 20 minutes, mixed with fresh DMEM at a 1:2 ratio, and the final serum concentration was adjusted to 10%. The mixed medium was used to culture primary human <t>fibroblasts</t> for 48 hours. (B) Fibroblasts were stained with senescence-associated β-gal, and the percentage of senescent cells was quantified by Image (J, C) Protein levels of P16 and P21 in fibroblasts were analyzed by western blotting. Densitometric analysis of protein bands were quantified by Image (J, D) RT-PCR analysis of RNA isolated from fibroblasts was performed to assess the expression of P16, P21 , P53 , TNFα , IL-6 , IL-1β , and MMP1 , with β-actin as the internal control. Con-SASP, SASP collected from control NHEKs; S.epi -SASP, SASP collected from NHEKs treated with 75μg/mL of ≤10kDa S.epi ; UVB-SASP, SASP collected from NHEKs treated with UVB; UVB+ S.epi -SASP, SASP collected from NHEKs treated with UVB and 75μg/mL of ≤10kDa S.epi . Data represent mean ± SEM with n = 3. All the experiments have been repeated three times. Statistical significances were analyzed by One-way ANOVA. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Normal Human Neonatal Dermal Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human neonatal dermal fibroblasts/product/ATCC
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    normal human neonatal dermal fibroblasts - by Bioz Stars, 2026-06
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    primary human dermal fibroblasts  (ATCC)
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    Staphylococcus epidermidis inhibits fibroblast senescence by inhibiting SASP secretion from UVB-irradiated keratinocytes. (A) Schematic representation of the experimental design. NHEKs were cultured to 90% confluence and then exposed to 10 mJ/cm 2 UVB for induction. Subsequently, 75μg/mL of ≤10kDa S. epi was added to coculture for 48 hours. The conditioned medium containing SASP factors was collected by centrifugation at 2, 000 rpm for 20 minutes, mixed with fresh DMEM at a 1:2 ratio, and the final serum concentration was adjusted to 10%. The mixed medium was used to culture primary human <t>fibroblasts</t> for 48 hours. (B) Fibroblasts were stained with senescence-associated β-gal, and the percentage of senescent cells was quantified by Image (J, C) Protein levels of P16 and P21 in fibroblasts were analyzed by western blotting. Densitometric analysis of protein bands were quantified by Image (J, D) RT-PCR analysis of RNA isolated from fibroblasts was performed to assess the expression of P16, P21 , P53 , TNFα , IL-6 , IL-1β , and MMP1 , with β-actin as the internal control. Con-SASP, SASP collected from control NHEKs; S.epi -SASP, SASP collected from NHEKs treated with 75μg/mL of ≤10kDa S.epi ; UVB-SASP, SASP collected from NHEKs treated with UVB; UVB+ S.epi -SASP, SASP collected from NHEKs treated with UVB and 75μg/mL of ≤10kDa S.epi . Data represent mean ± SEM with n = 3. All the experiments have been repeated three times. Statistical significances were analyzed by One-way ANOVA. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Primary Human Dermal Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human dermal fibroblasts/product/ATCC
    Average 99 stars, based on 1 article reviews
    primary human dermal fibroblasts - by Bioz Stars, 2026-06
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    fibroblast  (ATCC)
    99
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    Staphylococcus epidermidis inhibits fibroblast senescence by inhibiting SASP secretion from UVB-irradiated keratinocytes. (A) Schematic representation of the experimental design. NHEKs were cultured to 90% confluence and then exposed to 10 mJ/cm 2 UVB for induction. Subsequently, 75μg/mL of ≤10kDa S. epi was added to coculture for 48 hours. The conditioned medium containing SASP factors was collected by centrifugation at 2, 000 rpm for 20 minutes, mixed with fresh DMEM at a 1:2 ratio, and the final serum concentration was adjusted to 10%. The mixed medium was used to culture primary human <t>fibroblasts</t> for 48 hours. (B) Fibroblasts were stained with senescence-associated β-gal, and the percentage of senescent cells was quantified by Image (J, C) Protein levels of P16 and P21 in fibroblasts were analyzed by western blotting. Densitometric analysis of protein bands were quantified by Image (J, D) RT-PCR analysis of RNA isolated from fibroblasts was performed to assess the expression of P16, P21 , P53 , TNFα , IL-6 , IL-1β , and MMP1 , with β-actin as the internal control. Con-SASP, SASP collected from control NHEKs; S.epi -SASP, SASP collected from NHEKs treated with 75μg/mL of ≤10kDa S.epi ; UVB-SASP, SASP collected from NHEKs treated with UVB; UVB+ S.epi -SASP, SASP collected from NHEKs treated with UVB and 75μg/mL of ≤10kDa S.epi . Data represent mean ± SEM with n = 3. All the experiments have been repeated three times. Statistical significances were analyzed by One-way ANOVA. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Fibroblast, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    fibroblast - by Bioz Stars, 2026-06
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    Staphylococcus epidermidis inhibits fibroblast senescence by inhibiting SASP secretion from UVB-irradiated keratinocytes. (A) Schematic representation of the experimental design. NHEKs were cultured to 90% confluence and then exposed to 10 mJ/cm 2 UVB for induction. Subsequently, 75μg/mL of ≤10kDa S. epi was added to coculture for 48 hours. The conditioned medium containing SASP factors was collected by centrifugation at 2, 000 rpm for 20 minutes, mixed with fresh DMEM at a 1:2 ratio, and the final serum concentration was adjusted to 10%. The mixed medium was used to culture primary human <t>fibroblasts</t> for 48 hours. (B) Fibroblasts were stained with senescence-associated β-gal, and the percentage of senescent cells was quantified by Image (J, C) Protein levels of P16 and P21 in fibroblasts were analyzed by western blotting. Densitometric analysis of protein bands were quantified by Image (J, D) RT-PCR analysis of RNA isolated from fibroblasts was performed to assess the expression of P16, P21 , P53 , TNFα , IL-6 , IL-1β , and MMP1 , with β-actin as the internal control. Con-SASP, SASP collected from control NHEKs; S.epi -SASP, SASP collected from NHEKs treated with 75μg/mL of ≤10kDa S.epi ; UVB-SASP, SASP collected from NHEKs treated with UVB; UVB+ S.epi -SASP, SASP collected from NHEKs treated with UVB and 75μg/mL of ≤10kDa S.epi . Data represent mean ± SEM with n = 3. All the experiments have been repeated three times. Statistical significances were analyzed by One-way ANOVA. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Human Dermal Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human dermal fibroblasts/product/ATCC
    Average 99 stars, based on 1 article reviews
    human dermal fibroblasts - by Bioz Stars, 2026-06
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    neonatal human fibroblasts  (ATCC)
    99
    ATCC neonatal human fibroblasts
    Staphylococcus epidermidis inhibits fibroblast senescence by inhibiting SASP secretion from UVB-irradiated keratinocytes. (A) Schematic representation of the experimental design. NHEKs were cultured to 90% confluence and then exposed to 10 mJ/cm 2 UVB for induction. Subsequently, 75μg/mL of ≤10kDa S. epi was added to coculture for 48 hours. The conditioned medium containing SASP factors was collected by centrifugation at 2, 000 rpm for 20 minutes, mixed with fresh DMEM at a 1:2 ratio, and the final serum concentration was adjusted to 10%. The mixed medium was used to culture primary human <t>fibroblasts</t> for 48 hours. (B) Fibroblasts were stained with senescence-associated β-gal, and the percentage of senescent cells was quantified by Image (J, C) Protein levels of P16 and P21 in fibroblasts were analyzed by western blotting. Densitometric analysis of protein bands were quantified by Image (J, D) RT-PCR analysis of RNA isolated from fibroblasts was performed to assess the expression of P16, P21 , P53 , TNFα , IL-6 , IL-1β , and MMP1 , with β-actin as the internal control. Con-SASP, SASP collected from control NHEKs; S.epi -SASP, SASP collected from NHEKs treated with 75μg/mL of ≤10kDa S.epi ; UVB-SASP, SASP collected from NHEKs treated with UVB; UVB+ S.epi -SASP, SASP collected from NHEKs treated with UVB and 75μg/mL of ≤10kDa S.epi . Data represent mean ± SEM with n = 3. All the experiments have been repeated three times. Statistical significances were analyzed by One-way ANOVA. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Neonatal Human Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neonatal human fibroblasts/product/ATCC
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    neonatal human fibroblasts - by Bioz Stars, 2026-06
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    normal human neonatal foreskin fibroblasts ccd 1064sk  (ATCC)
    95
    ATCC normal human neonatal foreskin fibroblasts ccd 1064sk
    Staphylococcus epidermidis inhibits fibroblast senescence by inhibiting SASP secretion from UVB-irradiated keratinocytes. (A) Schematic representation of the experimental design. NHEKs were cultured to 90% confluence and then exposed to 10 mJ/cm 2 UVB for induction. Subsequently, 75μg/mL of ≤10kDa S. epi was added to coculture for 48 hours. The conditioned medium containing SASP factors was collected by centrifugation at 2, 000 rpm for 20 minutes, mixed with fresh DMEM at a 1:2 ratio, and the final serum concentration was adjusted to 10%. The mixed medium was used to culture primary human <t>fibroblasts</t> for 48 hours. (B) Fibroblasts were stained with senescence-associated β-gal, and the percentage of senescent cells was quantified by Image (J, C) Protein levels of P16 and P21 in fibroblasts were analyzed by western blotting. Densitometric analysis of protein bands were quantified by Image (J, D) RT-PCR analysis of RNA isolated from fibroblasts was performed to assess the expression of P16, P21 , P53 , TNFα , IL-6 , IL-1β , and MMP1 , with β-actin as the internal control. Con-SASP, SASP collected from control NHEKs; S.epi -SASP, SASP collected from NHEKs treated with 75μg/mL of ≤10kDa S.epi ; UVB-SASP, SASP collected from NHEKs treated with UVB; UVB+ S.epi -SASP, SASP collected from NHEKs treated with UVB and 75μg/mL of ≤10kDa S.epi . Data represent mean ± SEM with n = 3. All the experiments have been repeated three times. Statistical significances were analyzed by One-way ANOVA. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Normal Human Neonatal Foreskin Fibroblasts Ccd 1064sk, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human neonatal foreskin fibroblasts ccd 1064sk/product/ATCC
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    normal human neonatal foreskin fibroblasts ccd 1064sk - by Bioz Stars, 2026-06
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    primary human neonatal dermal fibroblasts  (ATCC)
    99
    ATCC primary human neonatal dermal fibroblasts
    Staphylococcus epidermidis inhibits fibroblast senescence by inhibiting SASP secretion from UVB-irradiated keratinocytes. (A) Schematic representation of the experimental design. NHEKs were cultured to 90% confluence and then exposed to 10 mJ/cm 2 UVB for induction. Subsequently, 75μg/mL of ≤10kDa S. epi was added to coculture for 48 hours. The conditioned medium containing SASP factors was collected by centrifugation at 2, 000 rpm for 20 minutes, mixed with fresh DMEM at a 1:2 ratio, and the final serum concentration was adjusted to 10%. The mixed medium was used to culture primary human <t>fibroblasts</t> for 48 hours. (B) Fibroblasts were stained with senescence-associated β-gal, and the percentage of senescent cells was quantified by Image (J, C) Protein levels of P16 and P21 in fibroblasts were analyzed by western blotting. Densitometric analysis of protein bands were quantified by Image (J, D) RT-PCR analysis of RNA isolated from fibroblasts was performed to assess the expression of P16, P21 , P53 , TNFα , IL-6 , IL-1β , and MMP1 , with β-actin as the internal control. Con-SASP, SASP collected from control NHEKs; S.epi -SASP, SASP collected from NHEKs treated with 75μg/mL of ≤10kDa S.epi ; UVB-SASP, SASP collected from NHEKs treated with UVB; UVB+ S.epi -SASP, SASP collected from NHEKs treated with UVB and 75μg/mL of ≤10kDa S.epi . Data represent mean ± SEM with n = 3. All the experiments have been repeated three times. Statistical significances were analyzed by One-way ANOVA. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Primary Human Neonatal Dermal Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human neonatal dermal fibroblasts/product/ATCC
    Average 99 stars, based on 1 article reviews
    primary human neonatal dermal fibroblasts - by Bioz Stars, 2026-06
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    Staphylococcus epidermidis inhibits fibroblast senescence by inhibiting SASP secretion from UVB-irradiated keratinocytes. (A) Schematic representation of the experimental design. NHEKs were cultured to 90% confluence and then exposed to 10 mJ/cm 2 UVB for induction. Subsequently, 75μg/mL of ≤10kDa S. epi was added to coculture for 48 hours. The conditioned medium containing SASP factors was collected by centrifugation at 2, 000 rpm for 20 minutes, mixed with fresh DMEM at a 1:2 ratio, and the final serum concentration was adjusted to 10%. The mixed medium was used to culture primary human fibroblasts for 48 hours. (B) Fibroblasts were stained with senescence-associated β-gal, and the percentage of senescent cells was quantified by Image (J, C) Protein levels of P16 and P21 in fibroblasts were analyzed by western blotting. Densitometric analysis of protein bands were quantified by Image (J, D) RT-PCR analysis of RNA isolated from fibroblasts was performed to assess the expression of P16, P21 , P53 , TNFα , IL-6 , IL-1β , and MMP1 , with β-actin as the internal control. Con-SASP, SASP collected from control NHEKs; S.epi -SASP, SASP collected from NHEKs treated with 75μg/mL of ≤10kDa S.epi ; UVB-SASP, SASP collected from NHEKs treated with UVB; UVB+ S.epi -SASP, SASP collected from NHEKs treated with UVB and 75μg/mL of ≤10kDa S.epi . Data represent mean ± SEM with n = 3. All the experiments have been repeated three times. Statistical significances were analyzed by One-way ANOVA. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Frontiers in Immunology

    Article Title: Staphylococcus epidermidis prevents UV-induced skin aging by suppressing TLR3-mediated senescence

    doi: 10.3389/fimmu.2026.1796085

    Figure Lengend Snippet: Staphylococcus epidermidis inhibits fibroblast senescence by inhibiting SASP secretion from UVB-irradiated keratinocytes. (A) Schematic representation of the experimental design. NHEKs were cultured to 90% confluence and then exposed to 10 mJ/cm 2 UVB for induction. Subsequently, 75μg/mL of ≤10kDa S. epi was added to coculture for 48 hours. The conditioned medium containing SASP factors was collected by centrifugation at 2, 000 rpm for 20 minutes, mixed with fresh DMEM at a 1:2 ratio, and the final serum concentration was adjusted to 10%. The mixed medium was used to culture primary human fibroblasts for 48 hours. (B) Fibroblasts were stained with senescence-associated β-gal, and the percentage of senescent cells was quantified by Image (J, C) Protein levels of P16 and P21 in fibroblasts were analyzed by western blotting. Densitometric analysis of protein bands were quantified by Image (J, D) RT-PCR analysis of RNA isolated from fibroblasts was performed to assess the expression of P16, P21 , P53 , TNFα , IL-6 , IL-1β , and MMP1 , with β-actin as the internal control. Con-SASP, SASP collected from control NHEKs; S.epi -SASP, SASP collected from NHEKs treated with 75μg/mL of ≤10kDa S.epi ; UVB-SASP, SASP collected from NHEKs treated with UVB; UVB+ S.epi -SASP, SASP collected from NHEKs treated with UVB and 75μg/mL of ≤10kDa S.epi . Data represent mean ± SEM with n = 3. All the experiments have been repeated three times. Statistical significances were analyzed by One-way ANOVA. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Neonatal human epidermal keratinocytes (NHEKs) and neonatal human fibroblasts were purchased from Lifeline Cell Technology.

    Techniques: Irradiation, Cell Culture, Centrifugation, Concentration Assay, Staining, Western Blot, Reverse Transcription Polymerase Chain Reaction, Isolation, Expressing, Control